This study focused on isolating and characterizing betulin (Lupan-3β,28-diol) from the bark of Acacia nilotica using bioassay-guided fractionation. Betulin was identified through Liebermann-Burchard testing and advanced spectroscopy techniques, including NMR and MS. The compound exhibited significant biological activities, including antioxidant, anti-inflammatory, and anticancer effects, with dose-dependent inhibitory effects observed in all assays. This research highlights the potential of betulin as a bioactive compound for therapeutic applications.
The project involved extracting the bark of Acacia nilotica using chloroform, ethyl acetate, and acetone, followed by bioassay-guided fractionation. The ethyl acetate fraction was purified through column chromatography to isolate betulin (AN-10). The compound was identified using Liebermann-Burchard test, NMR, and MS techniques. Biological activities such as antioxidant, anti-inflammatory, and anticancer effects were evaluated using various in vitro assays.
